This nano-liter droplet incubation array, or "StatDrop," provides a new method of creating single cell analysis arrays. The device is based on a reversed-order loading procedure, which results in an array of indexed stationary droplets with variable chemical composition. The array can support adherent and non-adherent cell cultures. StatDrop's simplicity to operate, minimal use of reagent, and portability make it convenient for uses such as creating multiplexed single cell performance assays for research and diagnostics.